Enrichment for late‐telophase cell populations using flow cytometry
نویسندگان
چکیده
منابع مشابه
Latent modeling of flow cytometry cell populations
Flow cytometry is a widespread single-cell measurement technology with a multitude of clinical and research applications. Interpretation of flow cytometry data is hard; the instrumentation is delicate and can not render absolute measurements, hence samples can only be interpreted in relation to each other while at the same time comparisons are confounded by inter-sample variation. Despite this,...
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Accurate analysis and quantification of different testicular cell populations are of central importance in studies of male reproductive biology. The traditional histomorphometric and immunohistochemical methods remain the gold standard in studying the complex dynamics of the testicular tissue. Through past years advances have been made in the application of flow cytometry for the rapid analysis...
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Multi-channel, high throughput experimental methodologies for flow cytometry are transforming clinical immunology and hematology, and require the development of algorithms to analyze the highdimensional, large-scale data. We describe the development of two combinatorial algorithms to identify rare cell populations in data from mice with acute promyelocytic leukemia. The flow cytometry data is c...
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AIM To evaluate a flow cytometry protocol that uses reference beads for the enumeration of live and dead bacteria present in a mixture. METHODS AND RESULTS Mixtures of live and dead Escherichia coli with live:dead concentration ratios varying from 0 to 100% were prepared. These samples were stained using SYTO 9 and propidium iodide and 6-μm reference beads were added. Bacteria present in live...
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ژورنال
عنوان ژورنال: Cytometry
سال: 2000
ISSN: 0196-4763,1097-0320
DOI: 10.1002/1097-0320(20001001)41:2<148::aid-cyto11>3.3.co;2-s